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How to avoid bacterial contamination during the use of Cell culture flasks?

Date：2023-09-25

Work in a Clean and Sterile Environment:

Perform all cell culture falsk work inside a certified biological safety cabinet (BSC) or laminar flow hood.

Ensure that the workspace is clean and disinfected before starting the cell culture procedure.

Personal Hygiene:

Wear appropriate personal protective equipment (PPE), including lab coats, gloves, and a mask or face shield.

Wash your hands thoroughly with soap and water before entering the cell culture area and after handling potentially contaminated materials.

Sterilize Equipment and Reagents:

Autoclave or sterilize all equipment, including cell culture flasks, pipettes, and media, before use.

Use sterile, disposable pipette tips and syringes to prevent contamination from reusable equipment.

Proper Aseptic Technique:

Practice aseptic technique to minimize the introduction of contaminants. This includes working swiftly and deliberately to minimize exposure of open culture vessels.

Flame or use an alcohol burner to sterilize tools such as forceps or scissors before using them.

Maintain Sterility of Media and Reagents:

Use sterile media, growth factors, and supplements.

Avoid introducing non-sterile objects (e.g., fingers, pipette tips) into media bottles or flasks.

Check Cell Line Authenticity:

Regularly verify the authenticity of your cell lines to ensure you are working with the correct cells.

Frequent Cleaning and Decontamination:

Clean and disinfect the biological safety cabinet or laminar flow hood before and after each use.

Routinely disinfect all surfaces and equipment in the cell culture area with appropriate disinfectants.

Avoid Overcrowding:

Do not overcrowd the incubator or the workspace with too many cell culture flasks. Proper spacing helps maintain airflow and reduces the risk of cross-contamination.

Minimize Lid Opening:

Limit the number of times you open cell culture flask lids to reduce exposure to the environment. When you do open them, keep the opening time as short as possible.

Change Gloves Frequently:

Change gloves whenever they become contaminated or between different steps of the cell culture process.

Monitor Cell Culture Health:

Regularly inspect your cell cultures under a microscope to check for any signs of contamination, such as bacterial growth or changes in cell morphology.

If contamination is suspected, isolate the affected culture immediately to prevent further spread.

Quarantine New Cell Lines:

Quarantine new cell lines for a period and perform regular checks for contamination before introducing them into your main cell culture area.

Follow Standard Operating Procedures (SOPs):

Develop and follow well-documented SOPs for your cell culture procedures to ensure consistency and compliance with practices.

Training and Education:

Ensure that all personnel working with cell cultures are properly trained in aseptic techniques and are aware of the importance of contamination prevention.

Keep Records:

Maintain detailed records of your cell culture procedures, including information about media, passage numbers, and any incidents of contamination. This documentation can help trace the source of contamination if it occurs.

For more information, please call us at +86-0571-87993109 or email us at hzbioland@126.com.

Tel：+86-0571-87993109

Email：hzbioland@126.com

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