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Date：2024-01-04
Sterile shake flasks play a crucial role in cell culture and fermentation processes, and their proper cleaning and storage are essential to maintain the integrity of experiments and prevent contamination. Here's a concise guide on how to clean and store sterile shake flasks after use:
Cleaning Sterile Shake Flasks:
1. Immediate Rinse:
After completing your culture or fermentation, promptly empty the contents of the shake flask.
Rinse the flask with an appropriate neutralizing solution or buffer to deactivate any remaining culture media.
2. Dismantle Components:
Disassemble any removable parts, such as caps and baffles, for thorough cleaning.
Pay attention to any nooks or crevices where residue may accumulate.
3. Pre-soak in Detergent:
Submerge the disassembled components in a mild detergent solution.
Allow the components to soak for a sufficient period, usually 30 minutes to an hour.
4. Scrubbing and Brushing:
Use a soft brush or sponge to scrub the interior of the flask, paying attention to areas that may trap contaminants.
Brush any removable components separately.
5. Autoclave or Sterilize:
Autoclave the cleaned components to ensure complete sterilization.
Follow manufacturer guidelines for autoclaving conditions.
6. Air-Dry:
Allow the components to air-dry in a clean environment.
Avoid using towels or wipes that may introduce contaminants.
Storing Sterile Shake Flasks:
1. Assemble Components:
Once dry, reassemble the shake flask, ensuring that all components are properly fitted.
2. Seal in Sterile Conditions:
Seal the flask using sterile materials, such as autoclaved aluminum foil or sterile caps.
Maintain aseptic conditions during this process to prevent contamination.
3. Label and Date:
Clearly label the flask with the contents, date of cleaning, and any other relevant information.
This helps in tracking usage and ensures that older flasks are not inadvertently used.
4. Store in a Clean Environment:
Store the sterile shake flask in a designated clean area, away from potential contaminants.
Avoid overcrowded storage spaces that may compromise sterility.
For more information, please call us at +86-0571-87993109 or email us at hzbioland@126.com.
Tel：+86-0571-87993109
Email：hzbioland@126.com
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